Journal
/
Developmental Biology
/
Targeted Laser Ablation in the Embryo of Saccharina latissima
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Targeted Laser Ablation in the Embryo of Saccharina latissima

Targeted Laser Ablation in the Embryo of Saccharina latissima

DOI:
10.3791/63518-v

•

06:30 min

•

March 11, 2022

•

, , ,
1UMR8227, CNRS / Sorbonne University, 2Univ Rennes, CNRS, Inserm, Biosit UAR 3480 US_S 018, MRic Core Facility

Chapters

  • 00:04Introduction
  • 00:47Image Acquisition for Selecting Embryos for Ablation and Monitoring Subsequent Growth
  • 01:31Laser Calibration
  • 02:48Laser Ablation
  • 04:21Monitoring the Growth of Irradiated Embryos
  • 04:49Results: Effect of Local Cellular Laser Ablation on the Saccharina latissima Embryo
  • 05:46Conclusion

Summary

Automatic Translation

Automatic Translation

The destruction of specific cells in the embryo is a powerful tool for studying cellular interactions involved in cell fate. The present protocol describes techniques for the laser ablation of targeted cells in the early embryo of the brown alga Saccharina latissima.

Tags

Keywords: Laser AblationSaccharina LatissimaEmbryogenesisBrown AlgaeCell FateCell InteractionImagingTime-lapse MicroscopyProtocol

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63518?article_type=v

Microbead Implantation in the Zebrafish Embryo
check_url/63518?article_type=v

Imaging Subcellular Structures in the Living Zebrafish Embryo
check_url/63518?article_type=v

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
check_url/63518?article_type=v

Surgical Ablation Assay for Studying Eye Regeneration in Planarians
check_url/63518?article_type=v

AFM and Microrheology in the Zebrafish Embryo Yolk Cell
check_url/63518?article_type=v

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)
check_url/63518?article_type=v

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
check_url/63518?article_type=v

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo
check_url/63518?article_type=v

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
check_url/63518?article_type=v

Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

Read Article