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Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
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Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Article DOI: 10.3791/63536-v • 09:20 min • February 1st, 2022
February 1st, 2022 •
1, 1
1Department of Biological Sciences, University of Pittsburgh

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Summary
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CUT&RUN and its variants can be used to determine protein occupancy on chromatin. This protocol describes how to determine protein localization on chromatin using single-cell uliCUT&RUN.

Tags

Single-cell Factor Localization Chromatin Ultra-Low Input Cleavage Under Targets And Release (NucleaseCUT&RUN) Technique Protein Localization ChIP-seq Manual 96-well Format High Cell Input CUT&RUN Optimization Low-cell Application Single-day Completion Biological Sample Rare And Low-abundance Samples Precious Patient Samples Antibody Testing Robust Antibody Procedure Demonstration Research Specialist Sorting Single Cells Binding Nuclei To Beads Magnetic Rack Blocking Buffer
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