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JoVE Journal Bioengineering
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Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

Article DOI: 10.3791/63544-v • 11:01 min • April 6th, 2022
April 6th, 2022 •
1, 1, 1, 3, 4, 2, 1
1Department of Biomedical Engineering, Stevens Institute of Technology, 2Department of Biomedical Engineering, Columbia University, 3Department of Cell Biology, State University of New York Downstate Medical Center, 4Department of Cardiothoracic Surgery, Stanford University

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The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.

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Imaging-guided Bioreactor Bioengineered Airway Tissue In Vitro Cultivation Microscopic Visualization Tissue Manipulation Procedures De-epithelization Airway Tissue Regeneration In Situ Imaging Tracheal Lumen Endogenous Epithelium Exogenous Cells Bioreactive Platform Disease Modeling Drug Screening Imaging-enabled Airway Tissue Bioreactor PhD Students Mohammad Mir Jiawen Chen Tube Lens Lens Tube Retaining Ring Lens Table Assembly Scientific CMOS Camera, C-mount Adapter Micromanager Software Photos And Videos Acquisition Optical Cage System Components Filter Lens
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