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JoVE Journal Bioengineering
Biorreactor guiado por imágenes para generar tejido biodiseñado de las vías respiratorias
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Biorreactor guiado por imágenes para generar tejido biodiseñado de las vías respiratorias

Article DOI: 10.3791/63544-v • 11:01 min • April 6th, 2022
April 6th, 2022 •
1, 1, 1, 3, 4, 2, 1
1Department of Biomedical Engineering, Stevens Institute of Technology, 2Department of Biomedical Engineering, Columbia University, 3Department of Cell Biology, State University of New York Downstate Medical Center, 4Department of Cardiothoracic Surgery, Stanford University

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El protocolo describe un biorreactor habilitado para imágenes que permite la eliminación selectiva del epitelio endógeno de la tráquea de la rata y la distribución homogénea de las células exógenas en la superficie de la luz, seguido de un cultivo in vitro a largo plazo de la construcción célula-tejido.

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Bioingeniería Número 182
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