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Journal / Immunology and Infection / Co-coltura di organoidi epiteliali murini…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Co-coltura di organoidi epiteliali murini dell'intestino tenue con cellule linfoidi innate
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Co-coltura di organoidi epiteliali murini dell'intestino tenue con cellule linfoidi innate

Article DOI: 10.3791/63554-v • 08:22 min • March 23rd, 2022
March 23rd, 2022 •
*1,2, *1,2,3, 1, 1
1Centre for Host-Microbiome Interactions, King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University
* These authors contributed equally

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Questo protocollo offre istruzioni dettagliate per stabilire organoidi murini dell'intestino tenue, isolare le cellule linfoidi innate di tipo 1 dalla lamina propria dell'intestino tenue murino e stabilire co-colture tridimensionali (3D) tra entrambi i tipi di cellule per studiare le interazioni bidirezionali tra le cellule epiteliali intestinali e le cellule linfoidi innate di tipo 1.

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Immunologia e infezione Numero 181
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