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Journal / Immunology and Infection / Cocultivo de organoides epiteliales del intestino …
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JoVE Journal Immunology and Infection
Cocultivo de organoides epiteliales del intestino delgado murino con células linfoides innatas
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Cocultivo de organoides epiteliales del intestino delgado murino con células linfoides innatas

Article DOI: 10.3791/63554-v • 08:22 min • March 23rd, 2022
March 23rd, 2022 •
*1,2, *1,2,3, 1, 1
1Centre for Host-Microbiome Interactions, King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University
* These authors contributed equally

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Este protocolo ofrece instrucciones detalladas para establecer organoides murinos del intestino delgado, aislar las células linfoides innatas tipo 1 de la lámina propia del intestino delgado murino y establecer cocultivos tridimensionales (3D) entre ambos tipos de células para estudiar las interacciones bidireccionales entre las células epiteliales intestinales y las células linfoides innatas tipo 1.

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Inmunología e Infección Número 181
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