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Neuroscience

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JoVE Journal Neuroscience
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route
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Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

Article DOI: 10.3791/63574-v • 05:35 min • May 31st, 2022
May 31st, 2022 •
1,2, 1,2, 1,2, 1,2
1Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 2GLIA Center, University of Yamanashi

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Summary
Automatic Translation

The protocol presented here allows the transplantation of induced pluripotent stem cell-derived human microglia (iPSMG) into the brain via a transnasal route in immunocompetent mice. The method for the preparation and transnasal transplantation of cells and the administration of cytokine mixture for the maintenance of iPSMG is shown.

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Transplantation Human Induced Pluripotent Stem Cell-derived Microglia Immunocompetent Mice Non-invasive Transnasal Route Protocol CSF1 Receptor Antagonist PLX5622 Brain Damage Neurodegenerative Diseases Bijay Parajuli Thawing Culture Media Centrifuge Transplantation Medium Cell Concentration Male Mice PLX Diet
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