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JoVE Journal Neuroscience
Transplante de Microglia Pluripotente Pluripotente Induzido Humano em Microglia De camundongos imunocompetentes através da Rota Transnasal Não Invasiva
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Transplante de Microglia Pluripotente Pluripotente Induzido Humano em Microglia De camundongos imunocompetentes através da Rota Transnasal Não Invasiva

Article DOI: 10.3791/63574-v • 05:35 min • May 31st, 2022
May 31st, 2022 •
1,2, 1,2, 1,2, 1,2
1Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 2GLIA Center, University of Yamanashi

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O protocolo aqui apresentado permite o transplante de microglia humana derivada de células-tronco pluripotentes induzidas (iPSMG) no cérebro através de uma rota transnasal em camundongos imunocompetentes. O método de preparação e transplante transnasal das células e a administração da mistura de citocinas para a manutenção do iPSMG é mostrado.

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Neurociência Edição 183
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