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Journal / Biochemistry / Rening av Ubiquitinated p53-proteiner från…
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JoVE Journal Biochemistry
Rening av Ubiquitinated p53-proteiner från däggdjursceller
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Rening av Ubiquitinated p53-proteiner från däggdjursceller

Article DOI: 10.3791/63602-v • 10:55 min • March 21st, 2022
March 21st, 2022 •
1, 1, 1
1Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

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Protokollet beskriver en steg-för-steg-metod för att rena ubiquitinerade proteiner från däggdjursceller med hjälp av p53-tumörsuppressorproteinet som exempel. Ubiquitinerade p53-proteiner renades från celler under stränga icke-denaturerings- och denatureringsförhållanden.

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Biokemi utgåva 181
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