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Zebrafish Corneal Wound Healing
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Zebrafish Corneal Wound Healing: dall'abrasione all'analisi di imaging della chiusura della ferita

Article DOI: 10.3791/63605-v • 08:19 min • March 1st, 2022
March 1st, 2022 •
1, 1, 1,2
1Institute of Biotechnology, HiLIFE, University of Helsinki, 2Institute for Neurosciences of Montpellier, Univ Montpellier

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Questo protocollo si concentra sul danneggiamento della superficie oculare del pesce zebra attraverso l'abrasione per valutare la successiva chiusura della ferita a livello cellulare. Questo approccio sfrutta una bava oculare per rimuovere parzialmente l'epitelio corneale e utilizza la microscopia elettronica a scansione per tracciare i cambiamenti nella morfologia cellulare durante la chiusura della ferita.

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Biologia Numero 181 Cornea epitelio abrasione bava oculare chiusura della ferita SEM zebrafish.
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