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JoVE Journal Bioengineering
formulere og karakterisere et eksosombasert dopaminbærersystem
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formulere og karakterisere et eksosombasert dopaminbærersystem

Article DOI: 10.3791/63624-v • 06:08 min • April 4th, 2022
April 4th, 2022 •
1,2, 3,4,5,6, 2,7, 2, 2, 8, 8, 3,4,5,6,9
1Vocational School of Health Services/İstanbul, Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering, Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology, Istinye University, 43D Bioprinting Design & Prototyping R&D Center, Istinye University, 5Stem Cell and Tissue Engineering R&D Center, Istinye University, 6Medicine Faculty, Istinye University, 7Vocational School of Health Services, Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital

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Her hadde vi som mål å oppnå en formulering ved dopaminbelastning av de isolerte eksosomene av stamceller fra Whartons gelémesenkymale stamceller. Eksosomisolering og karakterisering, legemiddelbelastning i de resulterende eksosomene og den cytotoksiske aktiviteten til den utviklede formuleringen er beskrevet i denne protokollen.

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Eksosombasert dopaminbærersystem ekstracellulære vesikler intercellulær last kommunikasjon kroppsvæsker blod-hjernebarriere nanobærersystem innkapsling av dopamin Whartons gelé mesenkymale stamceller legemiddelfrigivelse cytotoksisitetsanalyser fibroblast levedyktighet
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