JoVE Journal
Biochemistry
Biochemistry
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FDR制御ベースの非標的メタボロームにおける同定と定量化の統合ワークフロー
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Article
DOI:
10.3791/63625-v
•
05:35 min
•
September 20th, 2022
FDR制御ベースの非標的メタボロームにおける同定と定量化の統合ワークフロー
September 20th, 2022
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1Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, 2Chi-Biotech Co. Ltd.
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
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XY-MetaとmetaXを統合した非標的メタボロミクスワークフローを構築しました。このプロトコルでは、XY-Metaを使用してオープンアクセススペクトルリファレンスからデコイスペクトルライブラリを生成する方法を示し、FDR制御を行い、メタボロミクススペクトルを同定した後、metaXを使用して代謝産物を定量しました。