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Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy

Article DOI: 10.3791/63626-v • 08:03 min • March 25th, 2022
March 25th, 2022 •
*1,2, *1,2, 1,2, 1,2, 1,3, 4,5, 6, 1,2, 1,2,3,7
1Centro Nacional de Biologia Estrutural e Bioimagem - CENABIO, Universidade Federal do Rio de Janeiro, 2Instituto de Física, Programa de Pós-graduação Multidisciplinar em Física Aplicada, Universidade Federal do Rio de Janeiro, 3Instituto de Biofísica Carlos Chagas Filho, Programa de Pós-graduação em Ciências Biológicas Biofísica, Universidade Federal do Rio de Janeiro, 4Faculdade de Farmácia, Universidade Federal do Rio de Janeiro - Campus Macaé, 5Faculdade de Medicina, Programa de Pós-Graduação em Endocrinologia, Universidade Federal do Rio de Janeiro, 6Instituto de Ciências Exatas, Departamento de Física, Universidade Federal de Minas Gerais, 7Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro
* These authors contributed equally

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Summary
Automatic Translation

Here, an integrated protocol based on optical tweezers and defocusing microscopy is described to measure the rheological properties of cells. This protocol has wide applicability in studying the viscoelastic properties of erythrocytes under variable physio-pathological conditions.

Tags

Quantitative Analysis Viscoelastic Properties Red Blood Cells Optical Tweezers Defocusing Microscopy Mechanical Behavior Erythrocytes Viscoelastic Parameters Soft Glassy Features Physiological Conditions Pathological Conditions Single-cell Based Method Video References Form Factor Forces And Displacements Stresses And Scrapes Erythrocyte Surface Silicone Grease Rubber Ring Surface Coverslip Cell Culture Polystyrene Sphere Solution Sample Preparation Microscope OT System Sinusoidal
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