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Journal / Biology / Построение гомозиготных мутантов мигрирующей…
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Построение гомозиготных мутантов мигрирующей саранчи с использованием технологии CRISPR/Cas9
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Построение гомозиготных мутантов мигрирующей саранчи с использованием технологии CRISPR/Cas9

Article DOI: 10.3791/63629-v • 10:07 min • March 16th, 2022
March 16th, 2022 •
*1, *2,3, 2,3, 1, 2,3, 2,3, 2, 2
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 2Research Institute of Applied Biology, Shanxi University, 3School of Life Science, Shanxi University
* These authors contributed equally

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В этом исследовании представлена систематически оптимизированная процедура построения гомозиготных мутантов саранчи на основе рибонуклеазы CRISPR/Cas9, а также подробный метод криоконсервации и реанимации яиц саранчи.

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Ретракция выпуск 181 гомозиготные мутанты мигрирующая саранча CRISPR/Cas9 редактирование генома микроинъекция криоконсервация и реанимация яйцеклеток
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