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JoVE Journal Biology

カンジダ・アルビカンスにおける転写因子-DNA結合相互作用のゲノムワイドプロファイリング

Preparation of Cooling Stage Component and Construction of Water-Cooling Assembly

Journal (Biology)

Preparation of Cooling Stage Component and Construction of Water-Cooling Assembly

Dissection and Isolation of Murine Perivascular Adipose Tissue (PVAT)

Journal (Biology)

Dissection and Isolation of Murine Perivascular Adipose Tissue (PVAT)

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カンジダ・アルビカンスにおける転写因子-DNA結合相互作用のゲノムワイドプロファイリング:包括的なCUT&RUN法とデータ解析ワークフロー

Article DOI: 10.3791/63655-v • 07:48 min • April 1st, 2022

April 1st, 2022 •

Mohammad N. Qasim*^1,2, Ashley Valle Arevalo*^1,2, Akshay D. Paropkari*^1,2, Craig L. Ennis^1,2,4, Suzanne S. Sindi³, Clarissa J. Nobile^1,4, Aaron D. Hernday^1,4

¹Department of Molecular and Cell Biology, University of California, Merced, ²Quantitative and Systems Biology Graduate Program, University of California, Merced, ³Department of Applied Mathematics, University of California, Merced, ⁴Health Sciences Research Institute, University of California, Merced

* These authors contributed equally

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このプロトコルは、ヒト真菌病原体カン ジダ・アルビカンスにおけるヌクレアーゼ(CUT&RUN)を用いた標的下での切断および放出のための実験方法およびデータ分析ワークフローを記述する。

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生物学 カンジダ・アルビカンス CUT&RUN 転写因子ゲノムワイド結合法転写調節

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