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Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium
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Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

Article DOI: 10.3791/63658-v • 13:12 min • March 2nd, 2022
March 2nd, 2022 •
1, 2, 1,3
1Department of Ophthalmology, Louis J. Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, 2Earth Research Institute, University of California, Santa Barbara, 3Department of Developmental Biology, University of Pittsburgh School of Medicine

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Summary
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This protocol describes the methodology to genetically ablate the retinal pigment epithelium (RPE) using a transgenic zebrafish model. Adapting the protocol to incorporate signaling pathway modulation using pharmacological compounds is extensively detailed. A MATLAB platform for quantifying RPE regeneration based on pigmentation was developed and is presented and discussed.

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Nitroreductase Metronidazole-Mediated Ablation MATLAB Platform RpEGEN Zebrafish Retinal Pigment Epithelium Regeneration Molecular Pathways RPE Disease-related Mechanisms Versatility Degenerative Processes RPE Damage Pro-regenerative Factors Mammalian RPE Regeneration MTZ Solution Zebrafish Larvae Rpe65a:nsfB-eGFP Transgene Fluorescent Stereo Microscope
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