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Nitroreductase/metronidazolmedieret ablation og en MATLAB-platform (RpEGEN) til undersøgelse af regenerering af zebrafiskens retinale pigmentepitel
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Nitroreductase/metronidazolmedieret ablation og en MATLAB-platform (RpEGEN) til undersøgelse af regenerering af zebrafiskens retinale pigmentepitel

Article DOI: 10.3791/63658-v • 13:12 min • March 2nd, 2022
March 2nd, 2022 •
1, 2, 1,3
1Department of Ophthalmology, Louis J. Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, 2Earth Research Institute, University of California, Santa Barbara, 3Department of Developmental Biology, University of Pittsburgh School of Medicine

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Denne protokol beskriver metoden til genetisk at ablate retinal pigmentepitel (RPE) ved hjælp af en transgen zebrafiskmodel. Tilpasning af protokollen til at inkorporere signalvejsmodulering ved hjælp af farmakologiske forbindelser er udførligt detaljeret. En MATLAB-platform til kvantificering af RPE-regenerering baseret på pigmentering blev udviklet og præsenteres og diskuteres.

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Biologi udgave 181 zebrafisk retinal pigmentepitel nitroreductase metronidazol genetisk ablation regenerering pigmentering farmakologiske forbindelser RpEGEN
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