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Etablering av mänskliga lungorganoider och proximal differentiering för att generera mogna luftvägsorganoider
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Etablering av mänskliga lungorganoider och proximal differentiering för att generera mogna luftvägsorganoider

Article DOI: 10.3791/63684-v • 10:12 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3
1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong

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Protokollet presenterar en metod för att härleda mänskliga lungorganoider från primära lungvävnader, expandera lungorganoiderna och inducera proximal differentiering för att generera 3D- och 2D-luftvägsorganoider som troget fenoskopierar det mänskliga luftvägsepitelet.

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Biologi utgåva 181
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