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CRISPR-genredigeringsværktøj til analyse af mikroRNA-klynger
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CRISPR-genredigeringsværktøj til analyse af mikroRNA-klynger

Article DOI: 10.3791/63704-v • 10:40 min • April 25th, 2022
April 25th, 2022 •
1, 1, 1, 1
1Department of Microbiology & Molecular Cell Biology, Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School

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Denne protokol beskriver en high-throughput clustered regelmæssigt interspaced short palindromic repeats (CRISPR) genredigeringsarbejdsgang til mikroRNA-klyngenetværksanalyse, der muliggør hurtig generering af et panel af genetisk modificerede cellelinjer, der bærer unikke miRNA-klyngemedlemssletningskombinationer så store som 35 kb inden for et enkelt eksperiment.

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Biologi udgave 182 CRISPR inducerbar Cas9 microRNA knockout miR-888 klynge
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