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CRISPR Gene Editing Tool voor MicroRNA Cluster Network Analyse
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CRISPR Gene Editing Tool voor MicroRNA Cluster Network Analyse

Article DOI: 10.3791/63704-v • 10:40 min • April 25th, 2022
April 25th, 2022 •
1, 1, 1, 1
1Department of Microbiology & Molecular Cell Biology, Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School

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Dit protocol beschrijft een high-throughput clustered regular interspaced short palindromic repeats (CRISPR) genbewerkingsworkflow voor microRNA-clusternetwerkanalyse die de snelle generatie van een panel van genetisch gemodificeerde cellijnen met unieke miRNA-clusterliddeletiecombinaties zo groot als 35 kb binnen een enkel experiment mogelijk maakt.

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Biologie CRISPR induceerbare Cas9 microRNA knockout miR-888 cluster
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