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CRISPR-Gen-Editing-Tool für die MicroRNA-Cluster-Netzwerkanalyse
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CRISPR-Gen-Editing-Tool für die MicroRNA-Cluster-Netzwerkanalyse

Article DOI: 10.3791/63704-v • 10:40 min • April 25th, 2022
April 25th, 2022 •
1, 1, 1, 1
1Department of Microbiology & Molecular Cell Biology, Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School

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Dieses Protokoll beschreibt einen Hochdurchsatz-Cluster-Clustered-Regular-Interspaced-Short-Palindromic-Repeats-Gen-Editing-Workflow für die microRNA-Cluster-Netzwerkanalyse, der die schnelle Generierung eines Panels genetisch veränderter Zelllinien mit einzigartigen miRNA-Cluster-Deletionskombinationen von bis zu 35 kb innerhalb eines einzigen Experiments ermöglicht.

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Biologie Ausgabe 182 CRISPR induzierbares Cas9 microRNA Knockout miR-888 Cluster
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