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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Article DOI: 10.3791/63709-v • 09:57 min • July 25th, 2022
July 25th, 2022 •
1, 1, 1, 1, 1, 1, 2, 1,2, 1,2
1Dipartimento di Fisica, Politecnico di Milano, 2Institute for Photonics and Nanotechnologies (IFN-CNR)

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Summary
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We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

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Multiplex Chemical Imaging Broadband Stimulated Raman Scattering Microscopy Vibrational Spectrum Measurement Hyperspectral Microscopy Label-free Imaging Non-invasive Imaging Biological Sciences Biomedical Sciences Cells Imaging Tissues Imaging Broadband Optical Source Detection Chain OPR White Light Supercontinuum Photodiode Scanning Galvo Scanner Polymethyl Methacrylate Microbeads PMMA Microbeads Polystyrene Microbeads Aqueous Suspension Microscope Cover Slip Dimethyl Sulfoxide (DMSO)
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