Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / In situ Hybridisering kombineret med…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
In situ Hybridisering kombineret med immunhistokemi i kryosektionerede zebrafiskembryoner
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
Journal (Neuroscience)
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
Journal (Neuroscience)
Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
 
Click here for the English version

In situ Hybridisering kombineret med immunhistokemi i kryosektionerede zebrafiskembryoner

Article DOI: 10.3791/63715-v • 07:36 min • March 3rd, 2022
March 3rd, 2022 •
*1,2, *3, 2, 4, 2, 1, 2,3, 5, 1
1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver, hvordan man opnår billeder ved at kombinere in situ hybridisering og immunhistokemi af zebrafiskens embryonale sektioner. In situ hybridisering blev udført før kryosektion, efterfulgt af antistoffarvning. Det er nyttigt at detektere ekspressionsmønstre af to gener i zebrafisk, hvis der er mangel på antistoffer.

Tags

Denne måned i JoVE udgave 181 In situ hybridisering immunhistokemi zebrafisk mRNA antistof protein
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter