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Bioengineering
/
ヒトオルガノイドと臓器オンチップ技術を組み合わせて腸領域特異的機能をモデル化
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
Please note that all translations are automatically generated.
Click here for the English version.
ヒトオルガノイドと臓器オンチップ技術を組み合わせて腸領域特異的機能をモデル化
DOI:
10.3791/63724-v
•
10:56 min
•
May 05, 2022
•
Gauri Kulkarni*
1
,
Athanasia Apostolou*
1
,
Lorna Ewart
,
Carolina Lucchesi
,
Magdalena Kasendra
3
1
Emulate Inc. Boston
,
2
Center for Stem Cell and Organoid Medicine
,
Cincinnati Children’s Hospital Medical Center
,
3
Division of Pediatric General and Thoracic Surgery
,
Cincinnati Children’s Hospital Medical Center
Chapters
00:05
Introduction
01:24
Seeding of Organoid Fragments
03:14
Dynamic Culture of Intestine Chip
04:19
CYP450 Induction in the Intestine Chip
05:49
Sample Preparation for LC-MS Analysis
07:15
Stimulation with IFNγ
08:24
Results: CYP3A4 Induction and IFNγ Stimulation in the Intestine Chip
10:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
生検由来の腸オルガノイドと臓器オンチップ技術は、領域特異的な腸機能を再要約するためにミクロ生理学的プラットフォームに組み合わされます。
Tags
Human Organoids
Organ-on-a-chip
Intestinal Region-specific Functionality
Intestinal Epithelium
Pharmacokinetics
Drug-drug Interaction
Intestinal Epithelial Barrier Dysfunction
Intestinal Physiology
Pharmacodynamics
Seeding
Organoid Fragments
BMM Dissociation
Organoid Digestion
Advanced DMEM F12
Organoid Growth Medium
Coated Chips
Article
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