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Bioengineering

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JoVE Journal Bioengineering
Engineering og karakterisering af en optogenetisk model af det humane neuromuskulære kryds
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Engineering og karakterisering af en optogenetisk model af det humane neuromuskulære kryds

Article DOI: 10.3791/63759-v • 11:07 min • April 14th, 2022
April 14th, 2022 •
1, 1, 1,2, 1,3, 1,2,4
1Department of Biomedical Engineering, Columbia University, 2Department of Medicine, Columbia University, 3Gladstone Institutes, 4College of Dental Medicine, Columbia University

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Vi beskriver et reproducerbart, automatiseret og upartisk billeddannelsessystem til karakterisering af neuromuskulær krydsfunktion ved hjælp af humant konstrueret skeletmuskelvæv og optogenetiske motoneuroner. Dette system muliggør funktionel kvantificering af neuromuskulær forbindelse over tid og detekterer formindsket neuromuskulær funktion forårsaget af neurotoksiner og myasthenia gravis patient serum.

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