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Immunology and Infection

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JoVE Journal Immunology and Infection
Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
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Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms

Article DOI: 10.3791/63773-v • 07:28 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1,2
1Department of Microbiology, The Ohio State University, 2Department of Microbial Infection and Immunity, The Ohio State University

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Summary
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The present protocol describes the study of neutrophil-biofilm interactions. Staphylococcus aureus biofilms are established in vitro and incubated with peripheral blood-derived human neutrophils. The oxidative burst response from neutrophils is quantified, and the neutrophil localization within the biofilm is determined by microscopy.

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In Vitro Assays Human Neutrophils Staphylococcus Aureus Biofilms Interactions Visualize Quantify Standardized Protocols Assay Variability Donor Variation Biofilm Formation Streak Plate Technique Nutrient-rich Agar Plate PLL Coating Sterile Water Overnight Culture MEM-alpha Medium Glucose Supplementation Incubation Conditions
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