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Immunology and Infection

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JoVE Journal Immunology and Infection
Standardiserade in vitro-analyser för att visualisera och kvantifiera interaktioner mellan humana neutrofiler och Staphylococcus aureus-biofilmer
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Standardiserade in vitro-analyser för att visualisera och kvantifiera interaktioner mellan humana neutrofiler och Staphylococcus aureus-biofilmer

Article DOI: 10.3791/63773-v • 07:28 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1,2
1Department of Microbiology, The Ohio State University, 2Department of Microbial Infection and Immunity, The Ohio State University

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Detta protokoll beskriver studien av neutrofil-biofilminteraktioner. Staphylococcus aureus biofilmer etableras in vitro och inkuberas med perifera blod-härledda humana neutrofiler. Det oxidativa sprängresponsen från neutrofiler kvantifieras och neutrofillokaliseringen i biofilmen bestäms genom mikroskopi.

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Immunologi och infektion utgåva 184 biofilm neutrofiler ROS-produktion mikroskopi värd-patogeninteraktioner
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