Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

Mashanipalya G. Jagadeeshaprasad; Prem Kumar Govindappa; Amanda M. Nelson; John C. Elfar

doi:10.3791/63776

Journal

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Neuroscience

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Isolatie, kweek en karakterisering van primaire Schwann-cellen, keratinocyten en fibroblasten uit menselijke voorhuid

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

Isolatie, kweek en karakterisering van primaire Schwann-cellen, keratinocyten en fibroblasten uit menselijke voorhuid

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6,129 Views

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10:36 min

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March 23, 2022

DOI:

10.3791/63776-v

DOI:

10.3791/63776-v

•

10:36 min

March 23, 2022

•

6112 Views

Mashanipalya G. Jagadeeshaprasad¹, Prem Kumar Govindappa¹, Amanda M. Nelson², John C. Elfar¹

¹Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science (CORTS),The Pennsylvania State University College of Medicine ²Department of Dermatology,The Pennsylvania State University College of Medicine

Summary

De studie van wondgenezing geassocieerd met musculoskeletale schade vereist vaak de beoordeling van in vitro interacties tussen Schwann-cellen (SC's), keratinocyten en fibroblasten. Dit protocol beschrijft de isolatie, kweek en karakterisering van deze primaire cellen uit de menselijke voorhuid.