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JoVE Journal Chemistry
Ekstraksjon av ikke-protein aminosyrer fra cyanobakterier for væskekromatografi-tandem massespektrometri analyse
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Ekstraksjon av ikke-protein aminosyrer fra cyanobakterier for væskekromatografi-tandem massespektrometri analyse

Article DOI: 10.3791/63779-v • 08:14 min • December 9th, 2022
December 9th, 2022 •
1, 1, 2, 2, 1
1Hyphenated Mass Spectrometry Laboratory (HyMaS), University of Technology Sydney, 2School of Life Sciences, University of Technology Sydney

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Denne protokollen beskriver ekstraksjon av ikke-proteinaminosyrer fra biologiske matriser via trikloreddiksyre (TCA) proteinutfelling og syrehydrolyse før analyse ved bruk av væskekromatografi-tandem massespektrometri.

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Kjemi Utgave 190 Aminosyre proteinutfelling trikloreddiksyre (TCA) ikke-protein aminosyre BMAA cyanobakterier
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