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JoVE Journal Developmental Biology
Voorbereiding en morfologische analyse van Chick Cranial Neural Crest Cell Cultures
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Voorbereiding en morfologische analyse van Chick Cranial Neural Crest Cell Cultures

Article DOI: 10.3791/63799-v • 09:26 min • June 27th, 2022
June 27th, 2022 •
1, 2,3, 2,3, 2,3
1Department of Biology and Neuroscience Program, Hamline University, 2Department of Genetics, Cell Biology and Development, University of Minnesota, 3Developmental Biology Center, University of Minnesota

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Dit veelzijdige protocol beschrijft de isolatie van premigratory neural crest cells (NCC's) door de excisie van craniale neurale plooien van kuikenembryo's. Bij plating en incubatie komen migrerende NCC's tevoorschijn uit neurale vouwexplantaten, waardoor celmorfologie en migratie in een vereenvoudigde 2D-omgeving kunnen worden beoordeeld.

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Ontwikkelingsbiologie Nummer 184
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