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JoVE Journal Neuroscience
Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
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Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Article DOI: 10.3791/63800-v • 09:40 min • June 30th, 2022
June 30th, 2022 •
1, 1, 1, 1, 1, 1
1Department of Pharmacology and Physiology, Université de Montréal

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Summary
Automatic Translation

Nociceptor neurons and NK cells actively interact in an inflammatory context. A co-culture approach enables studying this interplay.

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Natural Killer Cells Nociceptor Neurons Interplay Co-culture Method Anti-tumor Function Elimination Of Injured Neurons Isolation And Culture Spleen PBS Homogenize Filter Cell Strainer Conical Tube Diluted Homogenate Hemocytometer Centrifuge Supernatant Resuspend RPMI 1640 Medium Magnetic Purification NK Cell Isolation Kit Flow Cytometry
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