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Biochemistry

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JoVE Journal Biochemistry
Un enfoque basado en la espectrometría de masas para identificar fosfoproteínas fosfatasas y sus interactores
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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Journal (Biochemistry)
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
 
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Un enfoque basado en la espectrometría de masas para identificar fosfoproteínas fosfatasas y sus interactores

Article DOI: 10.3791/63805-v • 10:17 min • April 29th, 2022
April 29th, 2022 •
1, 1,2
1Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, 2Norris Cotton Cancer Center

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Aquí, presentamos un protocolo para el enriquecimiento de fosfoproteínas fosfatasas endógenas y sus proteínas que interactúan a partir de células y tejidos y su identificación y cuantificación mediante proteómica basada en espectrometría de masas.

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Bioquímica Número 182
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