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Chapters
- 00:05Introduction
- 01:03Scar like Tissue in A Dish (SCAD) Preparation
- 02:50SCAD: Tissue Culture & Live Imaging of SCADs
- 04:23Tissue Harvesting and 2D/3D Immunofluorescence Staining
- 05:07Results: An Ex Situ Skin Scarring Assay to Visualize Scar Development
- 06:43Conclusion
Tags
SCAD AssayEx Situ Skin Scarring AssayFibroblast MigrationScar FormationWound RepairHigh-throughput ScreeningSkin ExplantsPost-natal Day Zero Or Day One SkinFull-thickness Dorsal Back SkinPanniculus Carnosus MuscleDMEM F-12 MediumHanks Balanced Salt Solution96-well PlateLive ImagingLow-melting Agarose Solution
