Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Elektroporationsmedieret levering af Cas9…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Elektroporationsmedieret levering af Cas9 Ribonucleoproteiner og mRNA til frisk isolerede primære musehepatocytter
Introduction to the Ultrasound Targeted Microbubble Destruction Technique
Journal (Bioengineering)
Introduction to the Ultrasound Targeted Microbubble Destruction Technique
Author Spotlight: Enhancing CAR-T Cell Function in Syngeneic Tumor Models (Video) | JoVE
Journal (Bioengineering)
Author Spotlight: Enhancing CAR-T Cell Function in Syngeneic Tumor Models (Video) | JoVE
 
Click here for the English version

Elektroporationsmedieret levering af Cas9 Ribonucleoproteiner og mRNA til frisk isolerede primære musehepatocytter

Article DOI: 10.3791/63828-v • 09:45 min • June 2nd, 2022
June 2nd, 2022 •
*1, *1, 1, 2, 1
1Department of Bioengineering, Clemson University, 2Godley Snell Research Center, Clemson University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver teknikker til isolering af primære musehepatocytter fra leveren og elektroporering af CRISPR-Cas9 som ribonukleoproteiner og mRNA for at forstyrre et terapeutisk målgen forbundet med en arvelig metabolisk sygdom i leveren. De beskrevne metoder resulterer i høj levedygtighed og høje niveauer af genmodifikation efter elektroporation.

Tags

Bioengineering udgave 184
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter