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Journal / Bioengineering / 갓 분리된 일차 마우스 간세포로의 Cas9 리보뉴클레오단백질 및 mRNA의 전기천공-매개 …
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
갓 분리된 일차 마우스 간세포로의 Cas9 리보뉴클레오단백질 및 mRNA의 전기천공-매개 전달
Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
Journal (Bioengineering)
Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
Journal (Bioengineering)
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
 
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갓 분리된 일차 마우스 간세포로의 Cas9 리보뉴클레오단백질 및 mRNA의 전기천공-매개 전달

Article DOI: 10.3791/63828-v • 09:45 min • June 2nd, 2022
June 2nd, 2022 •
*1, *1, 1, 2, 1
1Department of Bioengineering, Clemson University, 2Godley Snell Research Center, Clemson University
* These authors contributed equally

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이 프로토콜은 간으로부터 원발성 마우스 간세포를 단리하고 리보뉴클레오단백질 및 mRNA로서 CRISPR-Cas9를 전기천공하여 간장의 유전된 대사성 질환과 관련된 치료적 표적 유전자를 교란시키는 기술을 기술한다. 기술된 방법은 전기천공 후 높은 생존력과 높은 수준의 유전자 변형을 초래한다.

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생명공학 문제 184
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