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Journal / Bioengineering / Cas9 Ribonükleoproteinlerin ve mRNA'nın Yeni…
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Bioengineering

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JoVE Journal Bioengineering
Cas9 Ribonükleoproteinlerin ve mRNA'nın Yeni İzole Edilmiş Primer Fare Hepatositlerine Elektroporasyon Aracılı Verilmesi
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Cas9 Ribonükleoproteinlerin ve mRNA'nın Yeni İzole Edilmiş Primer Fare Hepatositlerine Elektroporasyon Aracılı Verilmesi

Article DOI: 10.3791/63828-v • 09:45 min • June 2nd, 2022
June 2nd, 2022 •
*1, *1, 1, 2, 1
1Department of Bioengineering, Clemson University, 2Godley Snell Research Center, Clemson University
* These authors contributed equally

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Bu protokol, primer fare hepatositlerini karaciğerden izole etme ve karaciğerin kalıtsal bir metabolik hastalığı ile ilişkili terapötik bir hedef geni bozmak için CRISPR-Cas9'u ribonükleoproteinler ve mRNA olarak elektroporasyon tekniklerini açıklar. Tarif edilen yöntemler, elektroporasyondan sonra yüksek canlılık ve yüksek düzeyde gen modifikasyonu ile sonuçlanır.

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