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JoVE Journal Developmental Biology
Isolering av hele celleprotein lysater fra mus ansiktsprosesser og kultivert palatal mesenchyme celler for fosfoprotein analyse
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Isolering av hele celleprotein lysater fra mus ansiktsprosesser og kultivert palatal mesenchyme celler for fosfoprotein analyse

Article DOI: 10.3791/63834-v • 07:26 min • April 1st, 2022
April 1st, 2022 •
*1, *1, 1
1Department of Craniofacial Biology, School of Dental Medicine, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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Protokollen presenterer en metode for å isolere hele celleprotein lysater fra dissekerte museembryo ansiktsprosesser eller dyrket mus embryonale palatal mesenchyme celler og utføre påfølgende vestlig blotting for å vurdere fosforylatert protein nivåer.

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Utviklingsbiologi Utgave 182 Kraniofacial utvikling fosforylering fosfoproteiner mus maksillære prosesser mus embryonale palatalmesenchyme celler
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