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Biochemistry

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JoVE Journal Biochemistry
Geavanceerde beeldvorming met hoge resolutie van virusassemblages in vloeistof en ijs
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Geavanceerde beeldvorming met hoge resolutie van virusassemblages in vloeistof en ijs

Article DOI: 10.3791/63856-v • 08:31 min • July 20th, 2022
July 20th, 2022 •
*1,2, *1, *1,3, 1,4, 1,4, 5, 6, 1, 7, 8, 2, 9, 1,5,6
1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University
* These authors contributed equally

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Hier worden protocollen beschreven om virusassemblages voor te bereiden die geschikt zijn voor vloeistof-EM- en cryo-EM-analyse op nanoschaal met behulp van transmissie-elektronenmicroscopie.

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Biochemie Nummer 185 transmissie-elektronenmicroscopie TEM liquid-EM cryo-EM adeno-geassocieerd virus SARS-CoV-2 rotavirus dubbellaagse deeltjes DLP's structurele biologie
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