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Faire progresser l’imagerie haute résolution des assemblages de virus dans le liquide et la glace
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Faire progresser l’imagerie haute résolution des assemblages de virus dans le liquide et la glace

Article DOI: 10.3791/63856-v • 08:31 min • July 20th, 2022
July 20th, 2022 •
*1,2, *1, *1,3, 1,4, 1,4, 5, 6, 1, 7, 8, 2, 9, 1,5,6
1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University
* These authors contributed equally

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Ici, des protocoles sont décrits pour préparer des assemblages de virus adaptés à l’analyse liquide-EM et cryo-EM à l’échelle nanométrique en utilisant la microscopie électronique à transmission.

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Biochimie numéro 185 microscopie électronique à transmission MET liquide-EM cryo-EM virus adéno-associé SARS-CoV-2 particules à double couche de rotavirus DLP biologie structurale
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