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High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health

Article DOI: 10.3791/63860-v • 11:40 min • April 28th, 2022
April 28th, 2022 •
*1, *1, 1, 1
1Division of Biomedical and Life Sciences, Lancaster University
* These authors contributed equally

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Summary
Automatic Translation

Gut microbes may positively or negatively impact the health of their host via specific or conserved mechanisms. Caenorhabditis elegans is a convenient platform to screen for such microbes. The present protocol describes high-throughput screening of 48 bacterial isolates for impact on nematode stress resistance, used as a proxy for worm health.

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High-Throughput Screening Microbial Isolates Caenorhabditis Elegans Health Oxidative Thermal Stress Resistance C.elegans Strains Bacterial Isolates Versatile And Scalable Approach Rapid Screening Conditions With Impact On C.elegans Health Study Of Mechanisms Of Health And Diseases Preclinical Screening Of Drugs Antiemetics Probiotics Nematode Mutant Knockdown Transgenic Disease Models Complex Interactions Host Microbe Interactions Metabolic Disorders Stress Handling Aging Contamination Prevention E.coli OP50 Bacteria Culture Preparation
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