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JoVE Journal Bioengineering
Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
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Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Article DOI: 10.3791/63862-v • 06:19 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 1,2, 1,2
1HKUST-Shenzhen Research Institute, 2Department of Chemistry, The Hong Kong University of Science and Technology

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Summary
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The present protocol describes a convenient approach to integrating optical trapping and surface-enhanced Raman spectroscopy (SERS) to manipulate plasmonic nanoparticles for sensitive molecular detection. Without aggregating agents, the trapping laser assembles plasmonic nanoparticles to enhance the SERS signals of target analytes for in situ spectroscopic measurements.

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Optical Trapping Plasmonic Nanoparticles In Situ Surface-enhanced Raman Spectroscopy SERS-active Nanoparticle Assembly Aggregation Agents Sensitive Detection Target Analytes Biomolecules Physiological Conditions Microfluidic System Trapping Laser Power Irradiation Time Silver Nanoparticle Concentration Optical Tweezer Microscope 532-nanometer Laser Beam Long-pass Dichroic Mirror Sample Chamber Backscattered Light Spectrometer Liquid-nitrogen-cooled Charge-coupled Device Camera Notch Filter
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