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Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction

Article DOI: 10.3791/63870-v • 09:15 min • July 12th, 2022
July 12th, 2022 •
1,2, 1,2
1Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus, 2Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus

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Summary
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Here we present a protocol for measuring absolute mitochondrial (mt)DNA copy number and mtDNA deletion heteroplasmy levels in single cells.

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Single-cell Mitochondrial DNA Copy Number Heteroplasmy Digital Droplet PCR Measurement Mutation Tissue Samples Single Cells Mitochondria Isolation Lysis Single Cell Sorting Master Mix PCR Plate Non-template Controls Input DNA Microliters Adhesive Plate Seal Shaker Centrifuge Droplet Generator
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