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Journal / Biochemistry / Oprensning og kvalitetskontrol af rekombinante…
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Biochemistry

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JoVE Journal Biochemistry
Oprensning og kvalitetskontrol af rekombinante septinkomplekser til cellefri rekonstitution
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Oprensning og kvalitetskontrol af rekombinante septinkomplekser til cellefri rekonstitution

Article DOI: 10.3791/63871-v • 11:50 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 2, 2, 3, 2, 1
1Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Institut Curie, Université PSL, Sorbonne Université

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In vitro rekonstitution af cytoskeletale proteiner er et vigtigt redskab til at forstå de grundlæggende funktionelle egenskaber ved disse proteiner. Dette papir beskriver en protokol til rensning og vurdering af kvaliteten af rekombinante septinkomplekser, som spiller en central rolle i celledeling og migration.

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