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Bioengineering

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JoVE Journal Bioengineering
Rask antistoffglykoengineering i kinesiske hamsterovarieceller
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Rask antistoffglykoengineering i kinesiske hamsterovarieceller

Article DOI: 10.3791/63872-v • 06:53 min • June 2nd, 2022
June 2nd, 2022 •
*1,2, *1,3, 1,4, 5, 5, 4, 1,2, 1
1Department of Chemical Engineering, Imperial College London, 2Imperial College Centre for Synthetic Biology, Imperial College London, 3BioPharm Process Research, GlaxoSmithKline Research and Development, 4Department of Life Science, Imperial College London, 5School of Chemical & Bioprocess Engineering, University College Dublin
* These authors contributed equally

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Glykosyleringsmønsteret til et antistoff bestemmer dens kliniske ytelse, og dermed vedvarer industriell og akademisk innsats for å kontrollere glykosylering. Siden typiske glykoengineeringskampanjer er tids- og arbeidskrevende, vil genereringen av en rask protokoll for å karakterisere effekten av glykosyleringsgener ved hjelp av forbigående silencing være nyttig.

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