JoVE Journal
Developmental Biology
Developmental Biology
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ヒト人工多能性幹細胞由来2型肺胞上皮細胞の3Dスフェアおよび2D気液界面培養物の生成
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Article
DOI:
10.3791/63875-v
•
07:02 min
•
April 15th, 2022
ヒト人工多能性幹細胞由来2型肺胞上皮細胞の3Dスフェアおよび2D気液界面培養物の生成
April 15th, 2022
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Rhiannon B. Werder*1,2,3, Jessie Huang*1,2, Kristine M. Abo1,2, Olivia T. Hix1,2, Kasey Minakin1,2, Konstantinos-Dionysios Alysandratos1,2, Carly Merritt1,2, Kayleigh Berthiaume1,2, Andrea B. Alber1,2, Claire L. Burgess1,2, Darrell N. Kotton*1,2, Andrew A. Wilson*1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
本プロトコールは、ヒト人工多能性幹細胞由来2型肺胞上皮様細胞(iAT2s)を記載する。これらの細胞は、3D培養において自己再生スフェアとして培養することも、気液界面(ALI)培養に適合させることもできる。