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Journal / Immunology and Infection / Kwantitatieve 3D-beeldvorming van trypanosoma…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Kwantitatieve 3D-beeldvorming van trypanosoma cruzi-geïnfecteerde cellen, slapende amastigoten en T-cellen in intacte geklaarde organen
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Kwantitatieve 3D-beeldvorming van trypanosoma cruzi-geïnfecteerde cellen, slapende amastigoten en T-cellen in intacte geklaarde organen

Article DOI: 10.3791/63919-v • 08:17 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 1, 1, 1,2
1Center for Tropical and Emerging Global Diseases, University of Georgia, 2Department of Cellular Biology, University of Georgia

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Het huidige protocol beschrijft lichtblad fluorescerende microscopie en geautomatiseerde software-ondersteunde methoden om prolifererende en slapende Trypanosoma cruzi-parasieten en T-cellen in intacte, gewiste organen en weefsels te visualiseren en nauwkeurig te kwantificeren. Deze technieken bieden een betrouwbare manier om behandelingsresultaten te evalueren en bieden nieuwe inzichten in interacties tussen parasieten en gastheer.

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Immunologie en infectie Nummer 184 Trypanosoma cruzi Chagas clearing immunostaining CUBIC light-sheet fluorescent microscopie
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