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Journal / Bioengineering / Udvikling af Drosophila melanogaster modeller til …
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Bioengineering

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JoVE Journal Bioengineering
Udvikling af Drosophila melanogaster modeller til billeddannelse og optogenetisk kontrol af hjertefunktionen
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Udvikling af Drosophila melanogaster modeller til billeddannelse og optogenetisk kontrol af hjertefunktionen

Article DOI: 10.3791/63939-v • 08:43 min • August 25th, 2022
August 25th, 2022 •
1, 1, 1, 1, 1,2, 1
1Department of Biomedical Engineering, Washington University in St. Louis, 2Department of Computer Science and Engineering, Washington University in St. Louis

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Den nuværende protokol beskriver dannelsen af Drosophila melanogaster , der udtrykker eNpHR2.0 eller ReaChR opsiner i hjertet til OCT-billeddannelse og optogenetisk hjertetempo. Detaljerede instruktioner til Drosophila OCT-billeddannelse og hjerteslagende modulering, herunder simulering af genoprettelig hjertestop, bradykardi og takykardi hos levende dyr på forskellige udviklingsstadier, rapporteres.

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Bioengineering Udgave 186 Drosophila melanogaster OCT optogenetik hjertestop bradykardi takykardi hjerte pacing opsin eNpHR2.0 ReaChR
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