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Journal / Genetics / التحقيق في التسبب في طفرة MYH7 Gly823Glu في…
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Genetics

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JoVE Journal Genetics
التحقيق في التسبب في طفرة MYH7 Gly823Glu في اعتلال عضلة القلب الضخامي العائلي باستخدام نموذج الماوس
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التحقيق في التسبب في طفرة MYH7 Gly823Glu في اعتلال عضلة القلب الضخامي العائلي باستخدام نموذج الماوس

Article DOI: 10.3791/63949-v • 03:45 min • August 8th, 2022
August 8th, 2022 •
*1,2, *3, 4, 4, 1,2, 1,2, 1,2, 2,5, 4, 1,2
1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine
* These authors contributed equally

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استنادا إلى عائلة اعتلال عضلة القلب الوراثي العائلي الموجودة في عملنا السريري ، أنشأنا نموذج فأر C57BL / 6N مع طفرة نقطية (G823E) في موضع الفأر MYH7 من خلال هندسة الجينوم بوساطة CRISPR / Cas9 للتحقق من هذه الطفرة.

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علم الوراثة ، العدد 186 ،
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