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Journal / Genetics / Undersöka patogenesen av MYH7-mutation Gly823Glu…
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JoVE Journal Genetics
Undersöka patogenesen av MYH7-mutation Gly823Glu i familjär hypertrofisk kardiomyopati med hjälp av en musmodell
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Undersöka patogenesen av MYH7-mutation Gly823Glu i familjär hypertrofisk kardiomyopati med hjälp av en musmodell

Article DOI: 10.3791/63949-v • 03:45 min • August 8th, 2022
August 8th, 2022 •
*1,2, *3, 4, 4, 1,2, 1,2, 1,2, 2,5, 4, 1,2
1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine
* These authors contributed equally

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Baserat på den familjära ärftliga kardiomyopatifamiljen som finns i vårt kliniska arbete skapade vi en C57BL/6N-musmodell med en punktmutation (G823E) vid musens MYH7-locus genom CRISPR/Cas9-medierad genomteknik för att verifiera denna mutation.

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Genetik utgåva 186
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