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Préparation de tissu myocardique humain pour la culture à long terme
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Préparation de tissu myocardique humain pour la culture à long terme

Article DOI: 10.3791/63964-v • 10:58 min • June 2nd, 2022
June 2nd, 2022 •
1,2, 1,2, 1,2,3, 4,5, 1,6, 1,2
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich

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Nous présentons un protocole pour la culture ex vivo de tissu myocardique ventriculaire humain. Il permet une analyse détaillée de la force de contraction et de la cinétique, ainsi que l’application de la pré- et post-charge pour imiter de plus près l’environnement physiologique in vivo .

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Médecine numéro 184 tissu myocardique humain culture ex vivo augmentation du débit contraction
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